N2 B27神經(jīng)分化培養(yǎng)基NDiff 227是一款成分確定、無(wú)血清、包含N2 & B27添加劑的*培養(yǎng)基，用于將單層貼壁生長(zhǎng)的小鼠胚胎干細(xì)胞向神經(jīng)方向分化。該款培養(yǎng)基上市時(shí)間超過10年，一共有超過47篇文獻(xiàn)使用。

 N2B27神經(jīng)分化培養(yǎng)基產(chǎn)品特征

成分確定、無(wú)血清的*培養(yǎng)基。

經(jīng)典配方，添加N2,B27。

只作用于小鼠細(xì)胞，有效促進(jìn)小鼠胚胎干細(xì)胞向神經(jīng)方向分化。

添加其他因子后，可以維持人/小鼠干細(xì)胞培養(yǎng)。

CellArtis Gibco B27神經(jīng)分化N2B27培養(yǎng)基神經(jīng)培養(yǎng)基及添加劑系列產(chǎn)品

CellArtis神經(jīng)細(xì)胞培養(yǎng)系列其他產(chǎn)品介紹

N2神經(jīng)培養(yǎng)基添加劑 

NDiff 細(xì)胞培養(yǎng)添加物

NDiff N2是成分*確定的、不添加血清的添加物，用于組織來源的神經(jīng)干細(xì)胞（tissue-derived neural stem (NS) cells）的分化獲取、維持培養(yǎng)、定向分化。Cellartis除了傳統(tǒng)型的NDiff N2，還提供不包含動(dòng)物源成分（animal-free (AF)）的NDiff N2。NDiff N2可以和與不含動(dòng)物源成分的RHB-Basal一起使用，從而開發(fā)適合使用者特殊細(xì)胞類型的培養(yǎng)基。

NDiff N2和NDiff N2-AF含有insulin, APO-transferrin (iron poor), progesterone, putrescine, 以及sodium selenite。

■ 產(chǎn)品特點(diǎn)

· Compley defined, serum-free formulation

· Animal-free (AF) version contains no components of animal origin

· Use with RHB-Basal medium for optimal performance

NDiff N2 is a proprietary, compley-defined, serum-free cell culture supplement for the derivation, maintenance, expansion, and/or differentiation of human and mouse stem cells and tissue-derived neural stem (NS) cells. Both traditional and animal-free (AF) versions are available. For optimal performance, NDiff N2 should be used with RHB-Basal medium; this medium is animal-component free and does not contain neuronal supplements, allowing medium customization to meet the specific requirements of your cells.

神經(jīng)干細(xì)胞培養(yǎng)基Neural Stem Cell Culture Media

RHB-A和RHB-Basal是兩款無(wú)血清、成分*確定的培養(yǎng)基，用于人類或小鼠神經(jīng)干細(xì)胞（NS）的定向分化獲取、維持培養(yǎng)、擴(kuò)增、誘導(dǎo)分化成神經(jīng)細(xì)胞等用途。

RHB-A，下一代N2B27神經(jīng)分化培養(yǎng)基。可用來培養(yǎng)擴(kuò)增貼壁的人或小鼠的神經(jīng)干細(xì)胞。在RHB-A中培養(yǎng)的神經(jīng)干細(xì)胞保持著穩(wěn)定的神經(jīng)干細(xì)胞分化發(fā)育成為成熟的神經(jīng)細(xì)胞的能力 (1-3) 。另外，RHB-A還用來定向分化小鼠胚胎干細(xì)胞(mouse ES)至神經(jīng)前體細(xì)胞 (neural precursors) (4-6)。

RHB‐Basal不包含生長(zhǎng)因子或是神經(jīng)細(xì)胞相關(guān)的添加物。因此，可以RHB‐Basal為基礎(chǔ)，通過添加客戶認(rèn)為需要的添加物來開發(fā)適合客戶的特殊類型細(xì)胞的培養(yǎng)基。

■ 產(chǎn)品特點(diǎn)

· RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells

· RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be customized by the addition of supplements

■ 產(chǎn)品應(yīng)用

· Derivation of mouse and human NS cells from ES cells and fetal and adult tissues

· Maintenance and propagation of adherent mouse and human NS cells

· Differentiation of mouse and human NS cells into functional neurons

· Differentiation of mouse ES cells to neuronal precursors

· Refer to the Data Sheet for additional examples of use

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